@article{oai:repository.naro.go.jp:00001583,
 author = {布目, 司 and NUNOME, Tsukasa},
 journal = {野菜茶業研究所研究報告, Bulletin of the National Institute of Vegetable and Tea Science},
 month = {Mar},
 note = {RAPD and AFLP markers in eggplant were developed and analyzed for polymorphisms between two eggplant lines, 'EPL1' and 'WCGR112-8'. A total of 1,224 random primers were screened by RAPD analysis for polymorphisms. Of them, 104 primers revealed polymorphisms, with 115 polymorphic fragments in total. In AFLP analysis, 43 of the 64 AFLP primer combinations used detected 104 polymorphic fragments. The frequencies of detected polymorphisms in RAPD and AFLP analyses were lower than those reported in other plant species. To develop microsatellite markers, a genomic library of eggplant was screened with dinucleotide and trinucleotide repeat probes. A total of 81 microsatellite markers were developed and analyzed for polymorphisms among 11 eggplant accessions and 11 accessions of 10 related species. Frequencies of microsatellites in eggplant were lower than those reported in other plant species. Using the RAPD, AFLP and microsatellite markers developed in the present study, a genetic linkage map was constructed on a F_2 population derived from a cross between 'EPL1' and 'WCGR112-8'. The linkage map consisted of 19 linkage groups (LGs) with 189 markers, spanning 807.3 cM in total. The observed number of LGs did not correspond to the expected number of 12 LGs for a comprehensive linkage map of eggplant. A major QTL that governs fruit shape was detected, and some QTLs that govern color development in fruits, stem, and calyx were also detected. These DNA markers would be useful for marker-assisted selection in eggplant breeding.},
 pages = {39--69},
 title = {ナスの分子遺伝マーカー開発と有用形質との連鎖解析に関する研究},
 volume = {4},
 year = {2005},
 yomi = {ヌノメ, ツカサ}
}