@article{oai:repository.naro.go.jp:00001556, author = {吉田, 克志 and YOSHIDA, Katsuyuki and 武田, 善行 and TAKEDA, Yoshiyuki}, journal = {野菜茶業研究所研究報告, Bulletin of the National Institute of Vegetable and Tea Science}, month = {Mar}, note = {Anthracnose, caused by Colletotrichum theae-sinensis (Miyake) Yamamoto, is one of the most serious diseases in the cultivation of tea (Camellia sinensis L.) in Japan. We have developed an assay method for anthracnose resistance in tea plants for disease resistance breeding. Conidia of C. theae-sinensis were suspended in an adhesive mixture consisting of potato-sucrose broth and methyl-cellulose 400 cP (3% w/v). Detached mature tea leaves were wounded crosswise by a screwdriver with adhesive conidial suspension. Inoculated leaves were cultivated on OASIS^(R) growing medium for two weeks under high humid conditions in a growth chamber. The degree of resistance to anthracnose was estimated by lesion size. The results of anthracnose assay of detached leaves related well to the natural occurrences of anthracnose in the tea field. This method is useful for tea breeding for anthracnose resistance.}, pages = {137--146}, title = {新しい付傷接種法を用いたチャ炭疸病抵抗性の検定法}, volume = {3}, year = {2004}, yomi = {ヨシダ, カツユキ and タケダ, ヨシユキ} }