@article{oai:repository.naro.go.jp:00001383,
 author = {室, 崇人 and MURO, Takato and 野口, 裕司 and NOGUCHI, Yuji and 森下, 昌三 and MORISHITA, Masami and 岡本, 大作 and OKAMOTO, Daisaku},
 journal = {北海道農業研究センター研究報告, Research BUlletin of the NARO Hokkaido Agricultural Research Center},
 month = {Mar},
 note = {We investigated a pretreatment method of spectroscopic analysis of samples for convenient evaluation of quercetin glycoside contents in onion varieties or breeding lines. We clarified the pretreatment process and processing time, and we showed that the sample preparation time and amount of reagent used can be reduced by approximately 90% and 95%, respectively, compared to those in the previously reported analytical protocol. The absorbance values, measured at 360 nm, of the extracts prepared by the procedure were highly correlated with the values of HPLC analysis (r=0.93), indicating that the simple evaluation method can be used for determining relative quercetin content of examined varieties. In addition, the pretreatment procedure was easy to carry out and had a high throughput. Thus, it was concluded that the simple evaluation method developed in this study is suitable for screening breeding lines, and it is expected to aid in the development of onion varieties with high quercetin glycoside contents.},
 pages = {1--229},
 title = {細断抽出によるタマネギケルセチン配糖体含量の簡易評価法},
 volume = {189},
 year = {2008},
 yomi = {ムロ, タカト and ノグチ, ユウジ and モリシタ, マサミ and オカモト, ダイサク}
}