{"created":"2023-05-15T13:37:36.376762+00:00","id":1266,"links":{},"metadata":{"_buckets":{"deposit":"44ebe048-7c9c-4ea8-bbb0-bce0cfeb90fb"},"_deposit":{"created_by":12,"id":"1266","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"1266"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00001266","sets":["87:597:592:126:186"]},"author_link":["2112"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"224","bibliographicPageStart":"199","bibliographicVolumeNumber":"110","bibliographic_titles":[{"bibliographic_title":"東北農業研究センター研究報告"},{"bibliographic_title":"Bulletin of TOHOKU Agricultural Research Center","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In an attempt to generate soybean plants resistant to Soybean dwarf virus (SbDV), which causes serious damage to soybean production in northern Japan, we transformed a construct containing inverted repeat-SbDV coat protein (CP) genes spaced by β-glucuronidase (GUS) sequences or sense CP gene into soybean somatic embryos via microprojectile bombardment. One and 6 independent T_0 plants were obtained, respectively. Former progenies exhibited SbDV resistance two months after inoculation by aphid. It was considered that these plants acquired the resistance to SbDV by an RNA-silencing-mediated process. Among the latter 6 transgenic lines, progenies of one line were subjected to further extensive analysis of SbDV-resistant response. In T_1 plants, the segregation of transgenes was confirmed, and three different lines were obtained. After inoculation of SbDV by the aphids, most T_2 plants of these lines remained symptomless. One transgenic line among the three lines acquired, presumably, resistance to SbDV by the RNA-silencing-mediated process from the threshold model. We transformed two different constructs into soybeans and produced SbDV-resistant transgenic soybeans mediated by RNA silencing.\n","subitem_description_type":"Abstract"},{"subitem_description":"本研究では、北日本において甚大な被害を起こしているダイズわい化病の病原ウイルスであるSoybean dwarf virus (SbDV) に抵抗性を持つ遺伝子組換えダイズの作出を行った。SbDVの外被タンパク質 (CP) 遺伝子の逆位反復配列とセンスSbDV CP 遺伝子を導入したダイズをそれぞれ1と6系統作出した。SbDV CP 遺伝子の逆位反復配列を導入して作出した後代系統はSbDV 抵抗性を示し、その抵抗性は逆位反復配列由来のshort interfering RNA (siRNA) によるRNAサイレンシング機構によるものと考えられた。センスSbDV CP 遺伝子を導入したダイズ6系統のうち、SbDV 抵抗性が高いと考えられた系統番号6の個体群について、詳細にSbDV抵抗性機構を解析した。系統番号6のT_1個体群で、導入遺伝子の分離が確認され、3つの分離系統を獲得した。これら3系統のT_2個体群はSbDV 抵抗性を示したが、そのうちの1系統は閾値モデルによるRNAサイレンシング機構によってSbDV 抵抗性が付与されたと考えられた。以上のように、２種類の異なる構造の遺伝子の導入を行い、RNAサイレンシング機構によるSbDV 抵抗性ダイズを作出した。\n","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00001233","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"独立行政法人 農業・食品産業技術総合研究機構 東北農業研究センター"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00001233","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-3379","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"藤郷, 誠"},{"creatorName":"トウゴウ, マコト","creatorNameLang":"ja-Kana"},{"creatorName":"TOGO, Makoto","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-09"}],"displaytype":"detail","filename":"tarc_report_No110p199-224p.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"tarc_report_No110p199-224p.pdf","url":"https://repository.naro.go.jp/record/1266/files/tarc_report_No110p199-224p.pdf"},"version_id":"5067f0a6-3c4b-46bb-b120-afe455b8389a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ダイズ","subitem_subject_scheme":"Other"},{"subitem_subject":"ダイズわい化病","subitem_subject_scheme":"Other"},{"subitem_subject":"RNAサイレンシング","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子組換え","subitem_subject_scheme":"Other"},{"subitem_subject":"抵抗性","subitem_subject_scheme":"Other"},{"subitem_subject":"soybean","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA silencing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SbDV","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"transgenic","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"resistance","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"遺伝子組換えによるダイズわい化病抵抗性ダイズの作出","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"遺伝子組換えによるダイズわい化病抵抗性ダイズの作出"},{"subitem_title":"Production of Soybean dwarf virus -resistant transgenic soybeans","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["186"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-22"},"publish_date":"2019-03-22","publish_status":"0","recid":"1266","relation_version_is_last":true,"title":["遺伝子組換えによるダイズわい化病抵抗性ダイズの作出"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T15:31:33.660881+00:00"}