{"created":"2023-05-15T13:37:33.617420+00:00","id":1212,"links":{},"metadata":{"_buckets":{"deposit":"ea162f2e-04c7-4ac3-b2f4-e9e739b7efa2"},"_deposit":{"created_by":12,"id":"1212","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"1212"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00001212","sets":["87:597:592:126:179"]},"author_link":["1420","2770","2063","2325","2771","713","773"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"37","bibliographicPageStart":"27","bibliographicVolumeNumber":"103","bibliographic_titles":[{"bibliographic_title":"東北農業研究センター研究報告"},{"bibliographic_title":"Bulletin of TOHOKU Agricultural Research Center","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Recently, demand for domestic bread-making wheat cultivars is increasing throughout Japan, including the Tohoku area. The goal of this study was to design DNA markers to help our Breeding Department develop new wheat cultivars with high-molecular-weight glutenin (HMWG) subunits responsible for high bread-making quality. HMWG subunits of storage proteins are highly related to bread-making quality. There are three Glu-1 loci, Glu-A1, Glu-B1, and Glu-D1, and several alleles from each locus have been characterized. Since the Glu-D1d allele responsible for producing the HMWG subunits \"5+10\" has the greatest positive effect on bread-making properties, a co-dominant DNA marker to be used for an effective identification of this allele was developed. By applying the marker to determine the presence or absence of the Glu-D1d allele in bread-making cultivars recently released from our institution, we showed that our PCR-based marker is more effective and accurate than the established SDS-PAGE method for HMWG subunit detection. In addition, the marker analysis revealed that the Glu-D1d allele was missing from the cultivar \"Yukichikara\", though this cultivar has been reported to carry the allele.\n","subitem_description_type":"Abstract"},{"subitem_description":"最近, パン用国産コムギに対する需要が全国的に伸びてきていることを受けて, 東北地方においても寒冷地向け早生・多収で製パン適性が高いコムギ品種の早期開発が求められている。そこで, 高い製パン適性を示す高分子量グルテニンサブユニットを持つコムギ新品種の育成に役立てるため, それらを簡易に判別できるDNA マーカーの開発を行った。小麦粉の生地物性に影響を与える貯蔵タンパク質である高分子量グルテニンは3つのGlu-1 座 (Glu-A1, Glu-B1 およびGlu-D1) に支配されており, そのうちGlu-D1 座の対立遺伝子Glu-D1d (「5+10」サブユニット) は生地物性を強化してパン体積を増大させることが知られている。そこで, Glu-D1d の有無を判別できる共優性のPCR 用DNA マーカーを開発した。また, 近年東北農業研究センターで育成されたパン用品種を中心に同マーカーによりGlu-D1d の有無の判定を行い, 従来のSDS-ポリアクリル電気泳動法と比較して, 有効であることを確認した。さらに, 当初Glu-D1d を持つと報告されていた「ゆきちから」はその遺伝子を持たないことを明らかにした。\n","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00001179","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"独立行政法人 農業・生物系特定産業技術研究機構 東北農業研究センター"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00001179","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-3379","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"石川, 吾郎"},{"creatorName":"イシカワ, ゴロウ","creatorNameLang":"ja-Kana"},{"creatorName":"ISHIKAWA, Goro","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"齋藤, 美香"},{"creatorName":"サイトウ, ミカ","creatorNameLang":"ja-Kana"},{"creatorName":"SAITO, Mika","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"伊藤, 裕之"},{"creatorName":"イトウ, ヒロユキ","creatorNameLang":"ja-Kana"},{"creatorName":"ITO, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"平, 将人"},{"creatorName":"タイラ, マサト","creatorNameLang":"ja-Kana"},{"creatorName":"TAIRA, Masato","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"前島, 秀和"},{"creatorName":"マエジマ, ヒデカズ","creatorNameLang":"ja-Kana"},{"creatorName":"MAEJIMA, Hidekazu","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"TANIGUCHI, Yoshinori","creatorNameLang":"en"},{"creatorName":"谷口, 義則","creatorNameLang":"ja"},{"creatorName":"タニグチ, ヨシノリ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"中村, 俊樹"},{"creatorName":"ナカムラ, トシキ","creatorNameLang":"ja-Kana"},{"creatorName":"NAKAMURA, Toshiki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-07"}],"displaytype":"detail","filename":"tarc_report_No103p27-37p.pdf","filesize":[{"value":"788.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"tarc_report_No103p27-37p.pdf","url":"https://repository.naro.go.jp/record/1212/files/tarc_report_No103p27-37p.pdf"},"version_id":"af70aba5-f8ed-4e78-ae0c-2bb8dd37b01d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"製パン適性","subitem_subject_scheme":"Other"},{"subitem_subject":"DNAマーカー","subitem_subject_scheme":"Other"},{"subitem_subject":"「5+10」サブユニット","subitem_subject_scheme":"Other"},{"subitem_subject":"Glu-D1d 対立遺伝子","subitem_subject_scheme":"Other"},{"subitem_subject":"「ゆきちから」","subitem_subject_scheme":"Other"},{"subitem_subject":"Bread-making quality","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA marker","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"\"5+10\" subunit","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Glu-D1d allele","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"\"Yukichikara\"","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"コムギ高分子量グルテニンサブユニット「5+10」を判別するPCR 用DNA マーカーの開発およびその東北地方向けパン用品種への適用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"コムギ高分子量グルテニンサブユニット「5+10」を判別するPCR 用DNA マーカーの開発およびその東北地方向けパン用品種への適用"},{"subitem_title":"Development of PCR-based DNA marker for identifying wheat high-molecular-weight glutenin subunits \"5+10\" and its applications to breeding of bread-making cultivars for the Tohoku region","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["179"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-22"},"publish_date":"2019-03-22","publish_status":"0","recid":"1212","relation_version_is_last":true,"title":["コムギ高分子量グルテニンサブユニット「5+10」を判別するPCR 用DNA マーカーの開発およびその東北地方向けパン用品種への適用"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-07-26T05:22:10.822477+00:00"}