2026-03-12T13:06:47Z https://repository.naro.go.jp/oai

oai:repository.naro.go.jp:00000222 2023-05-15T23:15:33Z 2:581:63 87:627:45

Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens SATO, Rie TESHIMA, Reiko KITTA, Kazumi Lang, Gang-Hua Kathleen, Schegg Kenneth, Blumenthal Leslie, Hicks Bénédicte, Labory-Carcenac David, Rouquié Rod_A., Herman Corinne, Herouet-Guicheney Gregory_S., Ladics Scott, McClain Lars_K., Poulsen Laura, Privalle Jason_M., Ward Nancy, Doerrer Jean-Baptiste, Rascle rice grain allergen proteomics multiplex immunodetection two-dimensional difference gel electrophoresis (2D-DIGE) rice grain allergen proteomics multiplex immunodetection two-dimensional difference gel electrophoresis (2D-DIGE) In rice, several allergens have been identified such as the non-specific lipid transfer protein-1, the α-amylase/trypsin-inhibitors, the α-globulin, the 33 kDa glyoxalase I (Gly I), the 52–63 kDa globulin, and the granule-bound starch synthetase. The goal of the present study was to define optimal rice extraction and detection methods that would allow a sensitive and reproducible measure of several classes of known rice allergens. In a three-laboratory ring-trial experiment, several protein extraction methods were first compared and analyzed by 1D multiplexed SDS-PAGE. In a second phase, an inter-laboratory validation of 2D-DIGE analysis was conducted in five independent laboratories, focusing on three rice allergens (52 kDa globulin, 33 kDa glyoxalase I, and 14–16 kDa α-amylase/trypsin inhibitor family members). The results of the present study indicate that a combination of 1D multiplexed SDS-PAGE and 2D-DIGE methods would be recommended to quantify the various rice allergens. journal article The Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA) Informa UK Limited, trading as Taylor & Francis Group 2016-07 VoR application/pdf Bioscience, Biotechnology, and Biochemistry 11 80 2198 2207 Bioscience, Biotechnology, and Biochemistry 0916-8451 https://repository.naro.go.jp/record/222/files/SC10201706260015_CCBYNCND_BBB80(11)p2198.pdf https://repository.naro.go.jp/records/222 eng 10.1080/09168451.2016.1206810 SC10201706260015 Bioscience, Biotechnology, and Biochemistry https://doi.org/10.1080/09168451.2016.1206810 © 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group