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        <identifier>oai:repository.naro.go.jp:00001498</identifier>
        <datestamp>2023-05-15T15:47:30Z</datestamp>
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          <dc:title>大麦・小麦α-およびβ-チオニンによる耐酸耐熱性細菌 Alicyclobacillus acidoterrestrisの制御</dc:title>
          <dc:title xml:lang="en">Control of Thermoacidophilic Alicyclobacillus acidoterrestris by Barley and Wheat α- and β-Thionins</dc:title>
          <jpcoar:creator>
            <jpcoar:creatorName>老田, 茂</jpcoar:creatorName>
            <jpcoar:creatorName xml:lang="ja-Kana">オイタ, シゲル</jpcoar:creatorName>
            <jpcoar:creatorName xml:lang="en">OITA, Shigeru</jpcoar:creatorName>
          </jpcoar:creator>
          <jpcoar:subject subjectScheme="Other">thionin</jpcoar:subject>
          <jpcoar:subject subjectScheme="Other">Alicyclobacillus acidoterrestris</jpcoar:subject>
          <jpcoar:subject subjectScheme="Other">antibacterial peptide</jpcoar:subject>
          <jpcoar:subject subjectScheme="Other">fruit juice</jpcoar:subject>
          <jpcoar:subject subjectScheme="Other">thermoacidophilic bacteria</jpcoar:subject>
          <jpcoar:subject xml:lang="en" subjectScheme="Other">thionin</jpcoar:subject>
          <jpcoar:subject xml:lang="en" subjectScheme="Other">Alicyclobacillus acidoterrestris</jpcoar:subject>
          <jpcoar:subject xml:lang="en" subjectScheme="Other">antibacterial peptide</jpcoar:subject>
          <jpcoar:subject xml:lang="en" subjectScheme="Other">fruit juice</jpcoar:subject>
          <jpcoar:subject xml:lang="en" subjectScheme="Other">thermoacidophilic bacteria</jpcoar:subject>
          <datacite:description descriptionType="Abstract">Heat-stable antimicrobial peptides, α- and β-hordothionins from barley and α-purothionin from wheat, inhibited the growth of thermoacidophilic spore-forming Alicyclobacillus acidoterrestris at a concentration of 5-10μg/ml. Viable cells of A. acidoterrestris decreased in satsuma mandarin juice and mixed fruit-vegetable juice containing 20μg/ml of α-purothionin, but the inhibition was relatively weak in 30% dilution of apple juice. α- and β-Hordothionins were extractable from barley seeds by 0.2 M citric and malic acids. The spoilage of satsuma mandarin juice by A. acidoterrestris was prevented by 3% addition of the barley extracts in the juice.</datacite:description>
          <datacite:description descriptionType="Abstract">大麦および小麦由来で熱安定性が高い抗菌ペプチドのα-およびβ-チオニンは，耐酸耐熱性細菌であるAlicyclobacillus acidoterrestrisの増殖を5-10μg/mlで阻害した. 20μg/mlの小麦α-チオニンを含むみかん果汁や果実・野菜ミックスジュース中でもA. acidoterrestrisの生菌数が減少したが，30%希釈のリンゴ果汁中では阻害がやや弱かった. 0.2 Mクエン酸およびリンゴ酸によって，大麦種子からα-およびβ-チオニンが抽出された. この大麦抽出液をみかん果汁へ3%添加したところ，A. acidoterrestrisによる果汁の腐敗が防止できた.</datacite:description>
          <dc:publisher>独立行政法人 農業技術研究機構 近畿中国四国農業研究センター</dc:publisher>
          <datacite:date dateType="Issued">2002-03-01</datacite:date>
          <dc:language>eng</dc:language>
          <dc:type rdf:resource="http://purl.org/coar/resource_type/c_6501">departmental bulletin paper</dc:type>
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          <jpcoar:identifier identifierType="DOI">https://doi.org/10.24514/00001457</jpcoar:identifier>
          <jpcoar:identifier identifierType="URI">https://repository.naro.go.jp/records/1498</jpcoar:identifier>
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          <jpcoar:sourceIdentifier identifierType="ISSN">1347-1244</jpcoar:sourceIdentifier>
          <jpcoar:sourceTitle>近畿中国四国農業研究センター研究報告</jpcoar:sourceTitle>
          <jpcoar:sourceTitle xml:lang="en">BULLETIN of THE NATIONAL AGRICULTURAL RESEARCH CENTER for WESTERN REGION</jpcoar:sourceTitle>
          <jpcoar:volume>1</jpcoar:volume>
          <jpcoar:pageStart>49</jpcoar:pageStart>
          <jpcoar:pageEnd>59</jpcoar:pageEnd>
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            <datacite:date dateType="Available">2019-03-15</datacite:date>
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