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落花生主要アレルゲンタンパク質のマウスにおける抗原性 Allergenicity of major allergenic peanut proteins on Balb/c mice 八巻, 幸二 ヤマキ, コウジ YAMAKI, Koji 高橋, 陽子 タカハシ, ヨウコ TAKAHASHI, Yoko peanut allergen Ara h1 Ara h2 Ara h3 antibody titer Using the simple purification method of the peanut antigenic proteins which we have developed before, we purified three kinds of allergenic protein from a peanut species and investigated the antigenicity against each protein and cross reactivity with the Balb/c mouse by alum immunization. Ara h1, Ara h2 and Ara h3, which was three kinds of major allergen, were injected in the abdominal cavity of the mouse with aluminum hydroxide gel for immunization. Two weeks later, blood was collected by cardiac puncture, the serum was measured antibody titer by enzyme linked immunosorbent assay. As a result, Ara h1 and h3 antibodies were increased more than Ara h2 antibody in IgG1 and IgG2a subclasses. It is reported that this Ara h2 shows a serious allergic symptom with the allergen of the peanut in human. However, there are increased antibody titer on h1 and h3 in this experiments with using the arum adjuvant. and it seems that it is important for the immunity model of the mouse to establish mouse allergic model of peanut allergens. In addition, it is suggested that the possibility of different antigenicity from human allergy disease to mouse allergic model. 国立研究開発法人 農業・食品産業技術総合研究機構 National Agriculture and Food Research Organization (NARO) 2018-03 jpn departmental bulletin paper VoR https://doi.org/10.24514/00000354 https://repository.naro.go.jp/records/377 10.24514/00000354 10.24514/00000354 2432-7891 農研機構研究報告 食品研究部門 Bulletin of the NARO,Food Research 2 35 40 https://repository.naro.go.jp/record/377/files/nfri_kenkyuhoukoku02_06.pdf application/pdf 1.2 MB 2018-05-16