This report describes the first collaborative expedition to collect Vietnamese plant genetic resources in the National Agriculture and Food Research Organization (NARO), Japan and the Plant Resources Center (PRC) of the Vietnamese Academy of Agricultural Science to establish an international research enterprise within the framework of the Plant Genetic Resources in Asia Phase 2 project. We conducted a field survey in the central highland of Vietnam (Gia Lai Province) between August 28 and September 4, 2018 and collected a total 113 accessions (16 of Amaranthus sp., 2 of Benincasa hispida, 33 of Cucumis melo, 10 of Cucumis sativus, 25 of Cucurbita moschata, 3 of Citrullus lanatus, 1 of Coccinia sp., 2 of Momordica charantia, 1 of Trichosanthes sp., 7 of Capsicum sp., 10 of Solanum sp., 1 of Solanum lycopersicum, 1 of Lablab purpureus, and 1 of Sorghum bicolor). All accessions were stored as seeds, and subsets were transferred to the Genetic Resources Center, NARO, Japan.