This paper reports on the conservation of crop landraces and crop wild relatives in Hyogo Prefecture in 2021. With the cooperation of the Hyogo-no-zairaishu-hozonkai, a society conserving crop landraces in Hyogo Prefecture, we received seeds of crop landraces from farmers and surveyed natural or artificially disturbed environments to collect seeds of crop wild relatives. We collected the seeds of 2 Mizuna landraces (Brassica rapa L. em. Metzg. subsp. nipposinica (L. H. Bailey) Hanelt), 3 accessions of turnip (Brassica rapa L. em. Metzg. subsp. rapa), 4 of mustard (Brassica juncea (L.) Czern. et Coss.), 2 of wild radish (Raphanus sativus L. var. raphanistroides Makino), 1 of soybean (Glycine max (L.) Merr.), and 1 of okra (Abelmoschus esculentus (L.) Moench) from 21 cultivation sites or habitats. The novelty of this report is the discovery of “Banshu-Takana,” a landrace of leaf mustard and “Banshu-Shiro-Mizuna,” a landrace of Mizuna as well as the introduction of naturally growing turnips and radishes into the National Agriculture and Food Research Organization (NARO) Genebank for the first time. After the seeds had multiplied, the NARO Genebank will preserve them for research and educational purposes (https://www.gene.affrc.go.jp/index_en.php).