A field survey was conducted to collect the salt-tolerant Leguminosae genetic resource, Vigna marina (Burm.) Merr., and their symbiotic rhizobia on the beaches of Tokunoshima Island, Okinoerabu Island, and Yoron Island, Kagoshima Prefecture, Japan, from November 18 to 22, 2019. In the field survey, 19 accessions of V. marina were collected. Root nodules were collected from 9 accessions of V. marina in order to isolate their symbiotic rhizobia, and soil samples were collected from the habitats of 6 accessions. All collected materials were conserved at the National Agriculture and Food Research Organization (NARO) Genebank of Japan.