A simple method for total RNA isolation from Aspergillus oryzae in solid-state culture (rice koji culture) was developed. A nucleic acid binding dye, aurintricarboxylic acid ammonium salt (ATA), was employed to prevent RNA from degradation by nuclease activity of rice koji culture. We also used cetyltrimethylammonium bromide (CTAB) to remove polysaccharides, which was mainly derived from rice grains. We successfully isolated total RNA from A. oryzae in solid-state culture. The RNA isolated by this method was available for Northern blot analysis and as a template of reverse transcription.
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アウリントリカルボン酸アンモニウムを用いた米麹からの麹菌全RNA抽出法の検討
nfri_report_No68p33-37p.pdf
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