The objective of this study was to investigate the effect of indoor feeding of large amounts of roughage on carcass composition and meat quality in steers. Twelve Japanese Black steers were randomly separated into hay-fed and concentrate-fed groups. The hay-fed steers were fed grass hay ad libitum and concentrate at 2.0 kg/day, whereas the concentrate-fed steers were fed concentrate ad libitum and grass hay at 1.5 kg/day. The steers were slaughtered at 28 months of age. Final body weight, daily gain, TDN intake, rib eye area, and rib thickness were greater in the concentrate-fed group than in the hay-fed group (p < 0.05). Lipid in muscle was higher in the concentrate-fed group (p < 0.10), but protein content was higher in the hay-fed group (p < 0.05). Drip loss in muscle after aging was lower in the hay-fed group than in the concentrate-fed group (p < 0.05). Although cooking loss after aging was lower in the hay-fed group, there was no significant difference between the groups (p > 0.05). Warner-Bratzler shear force in muscle was greater in the hay-fed group before aging (p < 0.05), but there was no significant difference from after aging (p > 0.05). Monounsaturated fatty acids in muscle were lower in the hay-fed group than in the concentrate-fed group, and saturated and polyunsaturated fatty acids showed no significant difference between the groups. Furthermore, n-3 polyunsaturated fatty acids were detected or increased in the hay-fed group compared with the concentrate-fed group. These results demonstrate that meat quality such as water-holding capacity and n-6/n-3 ratio may be improved by indoor feeding of grass hay.